Sequit: software for de novo peptide sequencing by matrix-assisted laser desorption/ionization post-source decay mass spectrometry

peer-reviewed · Rapid Communications in Mass Spectrometry · 2004

peer-reviewed · Rapid Communications in Mass Spectrometry · 2004. Rodion Demine et al. Peptide sequencing by mass spectrometry is gaining increasing importance for peptide chemistry and…
Date 2004-04-30
Type peer-reviewed
Venue Rapid Communications in Mass Spectrometry
Publisher Wiley
Contribution algorithm
DOI 10.1002/rcm.1420
Citations (OpenAlex) 31
Venue 2-year citedness 1.63

Abstract

Peptide sequencing by mass spectrometry is gaining increasing importance for peptide chemistry and proteomics. However, available tools for interpreting matrix-assisted laser desorption/ionization post-source decay (MALDI-PSD) mass spectra depend on databases, and identify peptides by matching experimental data with spectra calculated from database sequences. This severely obstructs the identification of proteins and peptides not listed in databases or of variations, e.g. mutated proteins. The development of a new computer program for database-independent peptide sequencing by MALDI-PSD mass spectrometry is reported here. This computer program was validated by the determination of the correct sequences for various peptides including sequences listed in the sequence databases, but also for peptides that deviate from database sequences or are completely artificial. This strategy should substantially facilitate the identification of novel or variant peptides and proteins, and increase the power of MALDI-PSD analyses in proteomics.

Authors

  1. Rodion Demine · Charité – Universitätsmedizin Berlin
  2. Peter Walden · Charité – Universitätsmedizin Berlin

Methods and tools

  • Sequit: Software for de novo peptide sequencing by MALDI post-source decay mass spectrometry.

Cites (7)

Cited by (2)

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