De novo peptide sequencing and quantitative profiling of complex protein mixtures using mass-coded abundance tagging
peer-reviewed · Nature Biotechnology · 2002
| Date | 2002-02-01 |
| Type | peer-reviewed |
| Venue | Nature Biotechnology |
| Publisher | Springer Science and Business Media LLC |
| Contribution | adjacent |
| DOI | 10.1038/nbt0202-163 |
| Citations (OpenAlex) | 231 |
| Venue 2-year citedness | 12.89 |
Abstract
Proteomic studies require efficient, robust, and practical methods of characterizing proteins present in biological samples. Here we describe an integrated strategy for systematic proteome analysis based on differential guanidination of C-terminal lysine residues on tryptic peptides followed by capillary liquid chromatography-electrospray tandem mass spectrometry. The approach, termed mass-coded abundance tagging (MCAT), facilitates the automated, large-scale, and comprehensive de novo determination of peptide sequence and relative quantitation of proteins in biological samples in a single analysis. MCAT offers marked advantages as compared with previously described methods and is simple, economic, and effective when applied to complex proteomic mixtures. MCAT is used to identify proteins, including polymorphic variants, from complex mixtures and measure variation in protein levels from diverse cell types.
Methods and tools
- MCAT: Mass-coded abundance tagging strategy for de novo peptide sequencing and quantitative profiling of complex protein mixtures.
Cites (4)
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Cited by (14)
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- 193 nm Ultraviolet Photodissociation of Imidazolinylated Lys-N Peptides for De Novo Sequencing (2012) crossref
- De novo sequencing of peptides by MS/MS (2010) crossref
- Peptide de Novo Sequencing Using 157 nm Photodissociation in a Tandem Time-of-Flight Mass Spectrometer (2010) crossref
- Rapid Validation of Mascot Search Results via Stable Isotope Labeling, Pair Picking, and Deconvolution of Fragmentation Patterns (2009) both
- Peptide de Novo Sequencing Facilitated by a Dual-Labeling Strategy (2005) crossref
- DeNovoID: a web-based tool for identifying peptides from sequence and mass tags deduced from de novo peptide sequencing by mass spectroscopy (2005) semanticscholar
- De novo sequence analysis of N‐terminal sulfonated peptides after in‐gel guanidination (2005) crossref
- Enhancing TOF/TOF-based de Novo Sequencing Capability for High Throughput Protein Identification with Amino Acid-Coded Mass Tagging (2005) crossref
- Two-Dimensional Mass Spectra Generated from the Analysis of 15N-Labeled and Unlabeled Peptides for Efficient Protein Identification and de novo Peptide Sequencing (2004) crossref
- Sequit: software for de novo peptide sequencing by matrix-assisted laser desorption/ionization post-source decay mass spectrometry (2004) crossref
- “De novo” peptide sequencing by MALDI-quadrupole-ion trap mass spectrometry: A preliminary study (2003) crossref
- Improved peptide sequencing using isotope information inherent in tandem mass spectra (2003) crossref
- Precise peptide sequencing and protein quantification in the human proteome through in vivo lysine-specific mass tagging (2003) crossref