Neuropeptidomics

10 workflows · 2003–2023

Neuropeptidomics: Neuropeptides and hormones, which are short, modified and easy to miss in a database search. 10 catalogued workflows and 10 papers.

Neuropeptides and hormones, which are short, modified and easy to miss in a database search.

Workflows 10
Papers 10
Authors 46
Active 2003-12-30 to 2023-02-01

Workflows (10)

  • Ascaris suum tissue neuropeptides (2003): De novo sequences novel neuropeptides directly from Ascaris suum tissue by MALDI TOF/TOF, profiling the tissue without the extraction losses and chemical degradation a workup would add.
  • Crustacean hyperglycemic hormone sequencing (2009): Combines bottom-up and top-down mass spectrometry to sequence the 72-residue crustacean hyperglycemic hormone of Cancer borealis de novo, the sequence having been unavailable for this species.
  • Sea urchin neuropeptidome hybrid de novo search (2010): Combines de novo sequencing with search of a translated genome to identify sea urchin neuropeptides, increasing the identification rate by 30%.
  • Tupaia belangeri neuropeptidome (2012): Integrated LC-FT-MS/MS, de novo sequencing and homology search identifies over a hundred neuropeptides in tree shrew brain, a species without a proteome.
  • CHH-family neuropeptide characterization (2012): High-definition de novo sequencing of crustacean hyperglycemic hormone family neuropeptides, large signalling peptides above 4 kDa whose functions cannot be read without both the sequence and the modifications.
  • Cancer borealis feeding neuropeptides (2021): Quantifies and images feeding-state neuropeptides in the crab Cancer borealis, with de novo sequencing yielding 69 new putative neuropeptides.
  • Callinectes sapidus neuropeptide sexual dimorphism (2021): Compares male and female blue crab neuropeptidomes by labelled quantitative MS, with de novo sequencing proposing hundreds of putative new neuropeptides.
  • Cancer borealis hemolymph feeding neuropeptides (2022): Profiles circulating neuropeptides in crab hemolymph over feeding and reports 96 putative new neuropeptide sequences found by de novo sequencing.
  • Insect adipokinetic hormone neuropeptidomics (2022): High-resolution LC-MS with de novo peptide sequencing to identify and confirm mature adipokinetic-hormone (AKH) neuropeptides, including novel sequences with post-translational hydroxyproline, across one cockroach, two moths and two flies, cross-checked against genome/transcriptome predictions.
  • Glycosylated crustacean neuropeptide discovery (2023): PEAKS de novo sequences carrying conserved neuropeptide-family motifs were used to build a putative neuropeptide database, which yielded glycoforms of nine putative novel neuropeptides in the blue crab Callinectes sapidus.

Papers (10)

Sequencing tools these papers used

PEAKS

Authors (46)

Adam D. Catherman, Alan G. Marshall, Amanda R. Buchberger, Antony O. Stretton, Ashley Phetsanthad, Bart Landuyt, Caroline Roycroft, Chenxi Jia, Christopher B. Lietz, Filomena Petruzziello, Geert Baggerman, Gerben Menschaert, Gerd Gäde, Gregor Rainer, Heather G. Marco, Henrik Wadensten, Huan He, Joanne Y. Yew, Jonathan V. Sweedler, Kellen DeLaney, Kylie Helfenbein, Laetitia Fouillen, Liliane Schoofs, Limei Hui, Lingjun Li, Mengzhou Hu, Michael P. Nusbaum, Mingming Ma, Neil L. Kelleher, Patsy S. Dickinson, Paul M. Thomas, Per E. Andren, Robert Kretz, Ruibing Chen, Sergei Dikler, Simone König, Tessa Hellenbrand, Tom T. M. Vandekerckhove, Walter Luyten, Weifeng Cao, Wenxin Wu, Wim Van Criekinge, Xiaoyue Jiang, Xiaozhe Zhang, Yang Liu (University of Wisconsin–Madison), Ying Ge

Where the work happened

Belgium, Germany, South Africa, Sweden, Switzerland, USA

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