HDPS
downstream-application · Hybrid de novo + database search
HDPS: downstream-application · Hybrid de novo + database search. Heuristic two-round sequence assembly strategy combining multi-enzyme and microwave-assisted acid hydrolysis, pNovo de novo peptide sequencing, and pFind homology…
Heuristic two-round sequence assembly strategy combining multi-enzyme and microwave-assisted acid hydrolysis, pNovo de novo peptide sequencing, and pFind homology database search with k-mer graph assembly and majority-vote error correction; achieved 100% sequence coverage and >98% amino-acid accuracy on full-length Ricin toxin A and B chains without a reference sequence, outperforming ALPS.
| Kind | downstream-application |
| Deep learning | no |
| Acquisition | DDA |
| Also known as | Heuristic De Novo Protein Sequencing |
| Family | Hybrid de novo + database search |
| Application area | Toxin identification (biodefense) |
Paper describing it
- Identification of Unknown Biological Toxin Proteins Using Mass Spectrometry: A Case Study on De Novo Sequencing of Ricin (2025, Toxins, peer-reviewed)