Me-Cou N-terminal derivatization de novo sequencing
adjacent · Chemical derivatization assisted
Me-Cou N-terminal derivatization de novo sequencing: adjacent · Chemical derivatization assisted. N-terminal derivatization with N-succinimidyl 7-methoxycoumarin-3-carboxylate (Me-Cou), which boosts low-mass b-ion yield so de novo sequencing can…
N-terminal derivatization with N-succinimidyl 7-methoxycoumarin-3-carboxylate (Me-Cou), which boosts low-mass b-ion yield so de novo sequencing can read the N-terminal residues, applied to untargeted peptidomics on LC-TIMS-qTOF/MS.
| Kind | adjacent |
| Deep learning | no |
| Acquisition | DDA |
| Family | Chemical derivatization assisted |
Papers describing it (3)
- de novo peptide sequencing method by using Coumarin-derivatized LC-MS/MS analysis (2025, Abstracts of the Annual Meeting of Japanese Society for Food Science and Technology, abstract)
- N-terminal Coumarin Derivatization-aided De Novo Peptide Sequencing and its Application to Peptidomics using LC-trapped Ion Mobility Spectrometry-qTOF/MS (2026, ChemRxiv, preprint)
- N-Terminal Coumarin Derivatization-Aided De Novo Peptide Sequencing and Its Application to Peptidomics Using LC-Trapped Ion Mobility Spectrometry-qTOF/MS (2026, Analytical Chemistry, peer-reviewed)