de novo peptide sequencing method by using Coumarin-derivatized LC-MS/MS analysis

abstract · Abstracts of the Annual Meeting of Japanese Society for Food Science and Technology · 2025

abstract · Abstracts of the Annual Meeting of Japanese Society for Food Science and Technology · 2025. Fumiya Honda et al. 【目的】De novoペプチドシーケンス法は,MS/MSスペクトルからアミノ酸配列を直接決定できるデータベース非依存的な手法であり,未知の低分子ペプチドの探索に有用である.しかしながら…
Date 2025-01-01
Type abstract
Venue Abstracts of the Annual Meeting of Japanese Society for Food Science and Technology
Publisher Japanese Society for Food Science and Technology
Contribution adjacent
DOI 10.3136/aamjsfst.72.0_115
Citations (OpenAlex) 0

Abstract

【目的】De novoペプチドシーケンス法は,MS/MSスペクトルからアミノ酸配列を直接決定できるデータベース非依存的な手法であり,未知の低分子ペプチドの探索に有用である.しかしながら, MS/MS解析ではフラグメントイオンの検出が複雑化しやすく,データベースとの照合が困難な7残基以下のペプチドでは配列決定が難しい.そこで本研究では,アミン誘導体化剤を利用した,新たなde novoペプチドシーケンス法の構築を目的とした.

Authors

  1. Fumiya Honda · Kyushu University
  2. Hui Luan · Kyushu University
  3. Yizhi Xiao · Kyushu University
  4. Risa Katagihara · Kyushu University
  5. Yumiko Toyama · Kyushu University
  6. Toshiro Matsui · Kyushu University
  7. Mitsuru Tanaka · Kyushu University

Methods and tools

  • Me-Cou N-terminal derivatization de novo sequencing: N-terminal derivatization with N-succinimidyl 7-methoxycoumarin-3-carboxylate (Me-Cou), which boosts low-mass b-ion yield so de novo sequencing can read the N-terminal residues, applied to untargeted peptidomics on LC-TIMS-qTOF/MS.

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