Multi-protease dual-fragmentation antibody sequencing

downstream-application · Protease strategy

Multi-protease dual-fragmentation antibody sequencing: downstream-application · Protease strategy. Direct de novo sequencing of monoclonal IgG from the purified product with a panel of complementary proteases and both stepped HCD and EThcD on every…

Direct de novo sequencing of monoclonal IgG from the purified product with a panel of complementary proteases and both stepped HCD and EThcD on every precursor, demonstrated on the anti-FLAG-M2 antibody.

Kind	downstream-application
Deep learning	no
Acquisition	DDA
Family	Protease strategy
Application area	Antibodyomics

Papers describing it (3)

• Mass spectrometry-based de novo sequencing of the anti-FLAG-M2 antibody using multiple proteases and a dual fragmentation scheme (2021, bioRxiv, preprint)
• Mass Spectrometry-Based De Novo Sequencing of Monoclonal Antibodies Using Multiple Proteases and a Dual Fragmentation Scheme (2021, Journal of Proteome Research, peer-reviewed)
• De novo antibody sequencing based on mass spectrometry (2024, thesis)

Authors (3)

Weiwei Peng, Matti F Pronker, Joost Snijder

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