N-terminal peptide isolation with CAF de novo sequencing
algorithm · Chemical derivatization assisted
N-terminal peptide isolation with CAF de novo sequencing: algorithm · Chemical derivatization assisted. Isolates protein N-terminal peptides on DITC resin, whether or not the N-terminus is blocked, then sequences them de novo by MALDI-TOF using…
Isolates protein N-terminal peptides on DITC resin, whether or not the N-terminus is blocked, then sequences them de novo by MALDI-TOF using chemically assisted fragmentation or MS/MS.
| Kind | algorithm |
| Deep learning | no |
| Acquisition | DDA |
| Family | Chemical derivatization assisted |
Paper describing it
- Selective isolation of N‐terminal peptides from proteins and their de novo sequencing by matrix‐assisted laser desorption/ionization time‐of‐flight mass spectrometry without regard to unblocking or blocking of N‐terminal amino acids (2008, Rapid Communications in Mass Spectrometry, peer-reviewed)