Simplified 18O labeling de novo sequencing
adjacent · Chemical labeling assisted
Simplified 18O labeling de novo sequencing: adjacent · Chemical labeling assisted. Adds H2 18O and trypsin directly to the digest so 18O is incorporated at peptide C-termini, marking y-ions to ease de novo sequencing without extra drying steps.
Adds H2 18O and trypsin directly to the digest so 18O is incorporated at peptide C-termini, marking y-ions to ease de novo sequencing without extra drying steps.
| Kind | adjacent |
| Deep learning | no |
| Acquisition | DDA |
| Family | Chemical labeling assisted |
Paper describing it
- A Simplified Method for Peptide De Novo Sequencing Using 18 O Labeling (2014, European Journal of Mass Spectrometry, peer-reviewed)