Chemical labeling assisted
8 methods · 1997–2017
Chemical labeling assisted: Isotopic or mass-coded labels introduced before the mass spectrometer, so one ion series can be told from the other by a known mass shift instead of by inference. The problem is eased in the sample, not the algorithm.
Isotopic or mass-coded labels introduced before the mass spectrometer, so one ion series can be told from the other by a known mass shift instead of by inference. The problem is eased in the sample, not the algorithm.
The earliest of its 8 methods is Rapid QTOF de novo sequencing (1997); 7 more have followed.
| Methods | 8 |
| Papers describing them | 8 |
| Authors | 36 |
| Active | 1997-06-15 to 2017-02-01 |
| Kinds | adjacent (7), algorithm |
| Acquisition | DDA (8) |
Methods (8)
Oldest first, by the paper that describes each one.
- Rapid QTOF de novo sequencing (1997): Nanoelectrospray, isotopic-labeling, and quadrupole/time-of-flight workflow for rapid de novo peptide sequencing.
- 18O ion trap de novo sequencing (1998): Ion-trap de novo peptide sequencing method using 18O labeling to distinguish fragment ion series.
- Isotopic N-terminal labeling de novo sequencing (2000): Isotopic N-terminal labeling with a fragmentation-directing moiety for protein quantitation and facilitated de novo sequencing.
- MCAT (2002): Mass-coded abundance tagging strategy for de novo peptide sequencing and quantitative profiling of complex protein mixtures.
- Reductive methylation de novo sequencing (2005): Reductive isotopic methylation strategy for de novo sequencing of neuropeptides.
- Dimethyl isotope labeling de novo sequencing (2010): Dimethyl isotope labeling-assisted workflow for de novo peptide sequencing.
- NeuCode y-ion annotation (2013): Two lysine isotopologues differing by 36 mDa are embedded metabolically, so every C-terminal fragment appears as a resolvable doublet and N-terminal ones do not. An algorithm reads that signature to label y-type ions at 93.2% accuracy, which among other uses lifts correct de novo identifications with PepNovo+ by 34%.
- NIPTL-Novo (2017): Non-isobaric peptide termini labeling assisted peptide de novo sequencing.
Papers describing them (8)
- Rapid de novo peptide sequencing by a combination of nanoelectrospray, isotopic labeling and a quadrupole/time-of-flight mass spectrometer (1997, Rapid Communications in Mass Spectrometry, peer-reviewed)
- De novo peptide sequencing in an ion trap mass spectrometer with 18O labeling (1998, Rapid Communications in Mass Spectrometry, peer-reviewed)
- Quantitation and Facilitated de Novo Sequencing of Proteins by Isotopic N-Terminal Labeling of Peptides with a Fragmentation-Directing Moiety (2000, Analytical Chemistry, peer-reviewed)
- De novo peptide sequencing and quantitative profiling of complex protein mixtures using mass-coded abundance tagging (2002, Nature Biotechnology, peer-reviewed)
- De Novo Sequencing of Neuropeptides Using Reductive Isotopic Methylation and Investigation of ESI QTOF MS/MS Fragmentation Pattern of Neuropeptides with N-Terminal Dimethylation (2005, Analytical Chemistry, peer-reviewed)
- Dimethyl isotope labeling assisted de novo peptide sequencing (2010, Journal of the American Society for Mass Spectrometry, peer-reviewed)
- Neutron-encoded Signatures Enable Product Ion Annotation From Tandem Mass Spectra (2013, Molecular & Cellular Proteomics, peer-reviewed)
- NIPTL-Novo: Non-isobaric peptide termini labeling assisted peptide de novo sequencing (2017, Journal of Proteomics, peer-reviewed)