TMPP N-terminal peptide de novo sequencing

algorithm · Chemical derivatization assisted

TMPP N-terminal peptide de novo sequencing: algorithm · Chemical derivatization assisted. Tags peptide N-termini with TMPP-Ac-OSu after metalloendopeptidase digestion and isolates them, giving MALDI spectra of N-terminal peptides clean enough to…

Tags peptide N-termini with TMPP-Ac-OSu after metalloendopeptidase digestion and isolates them, giving MALDI spectra of N-terminal peptides clean enough to sequence de novo.

Kind	algorithm
Deep learning	no
Acquisition	DDA
Family	Chemical derivatization assisted

Paper describing it

• A method for N-terminal de novo sequence analysis of proteins by matrix-assisted laser desorption/ionization mass spectrometry (2008, Analytical Biochemistry, peer-reviewed)

Authors (4)

Hiroki Kuyama, Kazuhiro Sonomura, Osamu Nishimura, Susumu Tsunasawa

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