ZipTip SPITC derivatization for MALDI-PSD de novo sequencing
algorithm · Chemical derivatization assisted
ZipTip SPITC derivatization for MALDI-PSD de novo sequencing: algorithm · Chemical derivatization assisted. Sulfonates tryptic peptides with 4-sulfophenyl isothiocyanate on C18 microZipTips so MALDI post-source decay spectra show only y ions, which…
Sulfonates tryptic peptides with 4-sulfophenyl isothiocyanate on C18 microZipTips so MALDI post-source decay spectra show only y ions, which are then read de novo to identify gel spots.
| Kind | algorithm |
| Deep learning | no |
| Acquisition | DDA |
| Family | Chemical derivatization assisted |
Paper describing it
- De novo sequencing of tryptic peptides sulfonated by 4‐sulfophenyl isothiocyanate for unambiguous protein identification using post‐source decay matrix‐assisted laser desorption/ionization mass spectrometry (2004, Rapid Communications in Mass Spectrometry, peer-reviewed)