CID and ETD mass spectrogram fusion based polypeptide de novo sequencing method
CN104034791A · Peking University · priority 2014
| Patent | CN104034791A ↗ |
| Status | Pending |
| Priority date | 2014-05-04 |
| Filed | 2014-05-04 |
| Published | 2014-09-10 |
| Assignee | Peking University |
Abstract
Translated from Chinese 本发明提供了一种基于CID与ETD质谱谱图融合的多肽从头测序方法，该方法包括以下步骤：1)对多肽进行N-末端正电荷衍生；2)对步骤1)所得衍生多肽分别进行CID质谱裂解与ETD质谱裂解；3)将步骤2)所得CID谱图与ETD谱图融合，得到CID-ETD融合谱图；4)对CID-ETD融合谱图进行解析。本发明还提供了所述方法在多肽从头测序中的应用。本发明的提供的多肽从头测序方法克服了单一的CID或ETD谱图无法提供完整多肽碎裂信息的缺陷，可以得到高可信度的多肽从头测序结果。 The present invention provides a polypeptide de novo sequencing method based on the fusion of CID and ETD mass spectrograms, the method comprising the following steps: 1) derivatizing the N-terminal positive charge of the polypeptide; 2) performing CID on the derived polypeptide obtained in step 1) respectively Mass spectrometry and ETD mass spectrometry; 3) merging the CID spectrum obtained in step 2) with the ETD spectrum to obtain a CID-ETD fusion spectrum; 4) analyzing the CID-ETD fusion spectrum. The invention also provides the application of the method in polypeptide de novo sequencing. The polypeptide de novo sequencing method provided by the present invention overcomes the defect that a single CID or ETD spectrum cannot provide complete fragmentation information of the polypeptide, and can obtain highly reliable polypeptide de novo sequencing results.
Why it is here
Peking University: de novo sequencing of N-terminally charge-derivatised peptides from fused CID and ETD spectra.
Inventors (3)
纪建国, 王青松, 安明瑞
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Catalogued papers it cites (1)
- High-Confidence de Novo Peptide Sequencing Using Positive Charge Derivatization and Tandem MS Spectra Merging (2013) added by the examiner
Matched from the patent’s 2 literature citations by the rules the paper citation graph uses.
Catalogued patents it cites (1)
- US7371514B2 Serial derivatization of peptides for de novo sequencing using tandem mass spectrometry
Cited by catalogued patents (1)
- CN106645456B Sequencing method for realizing monoclonal antibody drug amino acid sequence based on mass spectrometry technology
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- 上海市食品药品检验所 company: CN106645456A
- 中国科学院大连化学物理研究所 academic: CN111208237A
- 五邑大学 academic: CN114596912A
- 清华大学 academic: WO2021000786A1
- 萨默费尼根有限公司 company: CN110692118A
- 詹森生物科技公司 company: CN114829927A
Every family, of any applicant, that cites this one, as Google Patents lists them; an assignee is given its English name.