Sequencing method for realizing monoclonal antibody drug amino acid sequence based on mass spectrometry technology

CN106645456B · Shanghai Institute for Food and Drug Control · priority 2016

Patent CN106645456B (Shanghai Institute for Food and Drug Control, Active): The invention discloses a sequencing method for realizing a monoclonal antibody drug amino acid sequence based on a mass spectrometry technology, which utilizes mass…
Patent CN106645456B ↗
Status Active
Priority date 2016-10-13
Filed 2016-10-13
Published (granted) 2019-11-08
Assignee Shanghai Institute for Food and Drug Control
Current assignee SHANGHAI INSTITUTE FOR FOOD AND DRUG CONTROL; Shanghai Institute Of Drug Inspection
Claims PEAKS
Also published as CN106645456A

Abstract

The invention discloses a sequencing method for realizing a monoclonal antibody drug amino acid sequence based on a mass spectrometry technology, which utilizes mass spectrometry data dependency and full information tandem collection; the data obtained by the DDA acquisition of the mass spectrum is sequenced from the beginning by Peaks software and then MS is used E And collecting the obtained data to verify the amino acid sequence. The molecular weight of the monoclonal antibody reaches 150KDa, the determination of the amino acid sequence of the monoclonal antibody can be efficiently realized by adopting the invention, important means is provided for the research and development of monoclonal antibody medicines, the research of biological similar medicines and the quality control, and the de novo sequencing (de novo) of the monoclonal antibody medicine amino acid sequence is realized.

Why it is here

Shanghai Institute for Food and Drug Control: monoclonal antibody sequencing by PEAKS de novo sequencing of DDA data, verified with MSE data.

Inventors (4)

陈钢, 汪泓, 邵泓, 尹红锐

An inventor links to an author page only where the same person is evident, never on a name alone.

Catalogued patents it cites (1)

  • CN104034791A CID and ETD mass spectrogram fusion based polypeptide de novo sequencing method

Cited by catalogued patents (2)

  • CN117607307A A method and its application for de novo sequencing of monoclonal antibodies
  • CN113933420A Mass spectrum-based monoclonal antibody head-on sequencing method

Cited by (9 patent families, 9 organisations)

Every family, of any applicant, that cites this one, as Google Patents lists them; an assignee is given its English name.

Seen in the charts

Back to the full map

Back to top