Mass spectrum-based monoclonal antibody head-on sequencing method
CN113933420A · Shanghai Applied Protein Technology · priority 2021
| Patent | CN113933420A ↗ |
| Status | Pending |
| Priority date | 2021-09-30 |
| Filed | 2021-09-30 |
| Published | 2022-01-14 |
| Assignee | Shanghai Applied Protein Technology (printed Shanghai Applied Protein Technology Co Ltd) |
Abstract
The invention provides a mass spectrum-based monoclonal antibody de-novo sequencing method, which belongs to the field of biological medicine characterization, high-quality monoclonal antibody multi-enzymolysis liquid quality data are obtained through high performance liquid chromatography separation and mass spectrum detection, a secondary spectrum is subjected to de-novo sequencing and sequence assembly through sequencing software to obtain an amino acid sequence of a monoclonal antibody, and after a sequence output by the software is subjected to molecular weight verification and manual further analysis, the light and heavy chain sequence of the monoclonal antibody can reach 100% coverage rate and 99.5% or more amino acid accuracy, so that a method is provided for accurate and complete de-novo sequencing of an unknown monoclonal antibody in sequence to ensure the biological activity of the monoclonal antibody.
Why it is here
Shanghai Applied Protein Technology: monoclonal antibody de novo sequencing and assembly from multi-enzyme digests.
Inventors (3)
阮宏强, 薛冰冰, 李紫艺
An inventor links to an author page only where the same person is evident, never on a name alone.
Catalogued papers it cites (1)
- PEAKS DB: De Novo Sequencing Assisted Database Search for Sensitive and Accurate Peptide Identification (2012) added by the examiner
Matched from the patent’s 3 literature citations by the rules the paper citation graph uses.
Catalogued patents it cites (1)
- CN106645456B Sequencing method for realizing monoclonal antibody drug amino acid sequence based on mass spectrometry technology
Cited by catalogued patents (1)
- CN117607307A A method and its application for de novo sequencing of monoclonal antibodies
Cited by (1 patent families, 1 organisations)
- 深圳市药品检验研究院（深圳市医疗器械检测中心） academic: CN117607307A
Every family, of any applicant, that cites this one, as Google Patents lists them; an assignee is given its English name.