PEAKS DB: De Novo Sequencing Assisted Database Search for Sensitive and Accurate Peptide Identification
peer-reviewed · Molecular & Cellular Proteomics · 2012
peer-reviewed · Molecular & Cellular Proteomics · 2012. Jing Zhang et al. Many software tools have been developed for the automated identification of peptides from tandem mass…
| Date | 2012-04-01 |
| Type | peer-reviewed |
| Venue | Molecular & Cellular Proteomics |
| Publisher | ASBMB |
| Contribution | adjacent |
| DOI | 10.1074/mcp.M111.010587 |
| Citations (OpenAlex) | 1088 |
| Venue 2-year citedness | 4.69 |
Abstract
Many software tools have been developed for the automated identification of peptides from tandem mass spectra. The accuracy and sensitivity of the identification software via database search are critical for successful proteomics experiments. A new database search tool, PEAKS DB, has been developed by incorporating the de novo sequencing results into the database search. PEAKS DB achieves significantly improved accuracy and sensitivity over two other commonly used software packages. Additionally, a new result validation method, decoy fusion, has been introduced to solve the issue of overconfidence that exists in the conventional target decoy method for certain types of peptide identification software.
Methods and tools
- PEAKS DB: De-novo-assisted DB search
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