C-terminal de novo sequencing of peptides using oxazolone-based derivatization with bromine signature
peer-reviewed · Analytical Biochemistry · 2011
peer-reviewed · Analytical Biochemistry · 2011. Jong-Seo Kim et al. Due to almost identical chemical properties of C-terminal and side-chain carboxylic groups, selective…
| Date | 2011-12-01 |
| Type | peer-reviewed |
| Venue | Analytical Biochemistry |
| Publisher | Elsevier BV |
| Contribution | algorithm |
| DOI | 10.1016/j.ab.2011.08.011 |
| Citations (OpenAlex) | 19 |
Abstract
Due to almost identical chemical properties of C-terminal and side-chain carboxylic groups, selective C-terminal derivatization has been difficult. Although oxazolone-based C-terminal derivatization is the only selective C-terminal modification available, it has not been used widely because of its low derivatization efficiency. In this paper, an improved oxazolone chemistry for incorporation of Br signature to C-terminus is reported. MS/MS analysis of the brominated peptides led to a series of y ions with Br signature, facilitating de novo C-terminal sequencing.
Methods and tools
- Oxazolone C-terminal derivatization: Selective C-terminal derivatization, which is hard because C-terminal and side-chain carboxyls behave alike, improved in efficiency and given a bromine signature so the C-terminal fragment can be picked out and sequenced.