De novo peptide sequencing in an ion trap mass spectrometer with 18O labeling
peer-reviewed · Rapid Communications in Mass Spectrometry · 1998
peer-reviewed · Rapid Communications in Mass Spectrometry · 1998. Jun Qin et al. De novo peptide sequencing in an ion trap mass spectrometer coupled on-line with a capillary HPLC using 18O…
| Date | 1998-03-14 |
| Type | peer-reviewed |
| Venue | Rapid Communications in Mass Spectrometry |
| Publisher | Wiley |
| Contribution | adjacent |
| DOI | 10.1002/(SICI)1097-0231(19980314)12:5<209::AID-RCM141>3.0.CO;2-S |
| Citations (OpenAlex) | 49 |
| Venue 2-year citedness | 1.80 |
Abstract
De novo peptide sequencing in an ion trap mass spectrometer coupled on-line with a capillary HPLC using 18O labeling provides a viable alternative to the method using the combination of nanospray, 18O labeling and a quadrupole/time-of-flight mass spectrometer. Seven to sixteen amino acid residues can be sequenced from the liquid chromatography/randem mass spectrometry (LC/MS/MS) spectra. This approach combines the benefit of capillary LC and the high sensitivity of the ion trap operated in the MS/MS mode. The wide availability of the LCQ mass spectrometer makes this approach readily adaptable to the biological mass spectrometry community.
Methods and tools
- 18O ion trap de novo sequencing: Ion-trap de novo peptide sequencing method using 18O labeling to distinguish fragment ion series.
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- De novo protein sequence analysis of Macaca mulatta (2007) semanticscholar
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