Improved peptide sequencing using isotope information inherent in tandem mass spectra

peer-reviewed · Rapid Communications in Mass Spectrometry · 2003

peer-reviewed · Rapid Communications in Mass Spectrometry · 2003. W.R. Cannon et al. We demonstrate here the use of natural isotopic ‘labels’ in peptides to aid in the identification of peptides…
Date 2003-08-15
Type peer-reviewed
Venue Rapid Communications in Mass Spectrometry
Publisher Wiley
Contribution algorithm
DOI 10.1002/rcm.1119
Citations (OpenAlex) 22
Venue 2-year citedness 1.80

Abstract

We demonstrate here the use of natural isotopic ‘labels’ in peptides to aid in the identification of peptides with a de novo algorithm. Using data from ion trap tandem mass spectrometric (MS/MS) analysis of 102 tryptic peptides, we have analyzed multiple series of peaks within LCQ MS/MS spectra that ‘spell’ peptide sequences. Isotopic peaks from naturally abundant isotopes are particularly prominent even after peak centroiding on y- and b-series ions and lead to increased confidence in the identification of the precursor peptides. Sequence analysis of the MS/MS data is accomplished by finding sequences and subsequences in a hierarchical manner within the spectra.

Authors

  1. W.R. Cannon · Pacific Northwest National Laboratory
  2. K.D. Jarman · Pacific Northwest National Laboratory

Methods and tools

  • Hierarchical subsequence de novo scoring (PNNL): PNNL de novo approach that finds sequences and subsequences hierarchically in a reduced spectrum graph, using multiple ion series and natural isotope peaks, and ranks candidates by the probability of random matches.

Cites (8)

Cited by (7)

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