Expanding the repertoire of conopeptides from the venom of the vermivorous snail Conus regius
peer-reviewed · Toxicon · 2025
| Date | 2025-08-23 |
| Type | peer-reviewed |
| Venue | Toxicon |
| Publisher | Elsevier BV |
| Contribution | downstream-application |
| DOI | 10.1016/j.toxicon.2025.108551 |
| Citations (OpenAlex) | 1 |
| Venue 2-year citedness | 1.78 |
Abstract
This study presents a comprehensive proteomic characterization of Conus regius venom. Venom samples were fractionated using reversed-phase chromatography and analyzed by Q-TOF mass spectrometry. Spectral data were processed with Peaks Studio V7.0 and searched against a Conus protein database. A total of 520 peptide and protein sequences were identified, with most corresponding to conopeptides. Among these, eight presented similarities to disulfide-poor conopeptides of the Conantokin, ConoGay, and ConoCAP classes, as well as two sequences not classified in the literature. Additionally, 284 peptides showed similarity to conotoxins from 13 gene superfamilies (M, A, T, P, S, I1, Conodipine, O1, Insulin, G2, O2, Q, and H), while 102 sequences were linked to undetermined superfamilies. Of the identified sequences, 21 had been previously described in C. regius venom, while many others shared similarities with conotoxins from other Conus species. Most conotoxins identified were associated with the M and A superfamilies, suggesting their predominance in the venom. This study expands the known repertoire of C. regius venom components and provides valuable insights into its molecular diversity, supporting future research into its biotechnological potential.
Methods and tools
- Conus regius conopeptidome: Reversed-phase LC + Q-TOF MS/MS with Peaks Studio v7 de novo sequencing to identify 520 conopeptides from the vermivorous cone snail Conus regius, spanning 13 conotoxin gene superfamilies and eight disulfide-poor peptide classes: the first molluscan entry in the venomics lane.
Methods it uses
- PEAKS: Commercial DP-based de novo