Mass Spectrometric Protein Sequencing de Novo: The Full Determination of a 13.7 kDa Camelid Nanobody Sequence by MALDI Top-Down-Sequencing

abstract · PubMed Central · 2010

abstract · PubMed Central · 2010. Anja Resemann et al.
Date 2010-09-01
Type abstract
Venue PubMed Central
Contribution downstream-application
Link https://www.ncbi.nlm.nih.gov/pmc/articles/2918022
Citations (OpenAlex) 0

Authors

  1. Anja Resemann
  2. Dirk Wunderlich
  3. Jens Fuchser
  4. Detlev Suckau

Methods and tools

  • Camelid VHH top-down sequencing: Determines the primary structure of a 13.6 kDa camelid single heavy chain antibody by MALDI-TOF/TOF top-down analysis, recovering most of the sequence from the intact protein rather than from peptides.

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