Partial De Novo Sequencing and Unusual CID Fragmentation of a 7 kDa, Disulfide-Bridged Toxin
peer-reviewed · Journal of the American Society for Mass Spectrometry · 2012
| Date | 2012-05-01 |
| Type | peer-reviewed |
| Venue | Journal of the American Society for Mass Spectrometry |
| Publisher | American Chemical Society (ACS) |
| Contribution | downstream-application |
| DOI | 10.1007/s13361-012-0350-x |
| Citations (OpenAlex) | 15 |
| Venue 2-year citedness | 2.84 |
Abstract
A 7 kDa toxin isolated from the venom of the Texas coral snake (Micrurus tener tener) was subjected to collision-induced dissociation (CID) and electron-transfer dissociation (ETD) analyses both before and after reduction at low pH. Manual and automated approaches to de novo sequencing are compared in detail. Manual de novo sequencing utilizing the combination of high accuracy CID and ETD data and an acid-related cleavage yielded the N-terminal half of the sequence from the reduced species. The intact polypeptide, containing 3 disulfide bridges produced a series of unusual fragments in ion trap CID experiments: abundant internal amino acid losses were detected, and also one of the disulfide-linkage positions could be determined from fragments formed by the cleavage of two bonds. In addition, internal and c-type fragments were also observed.
Methods and tools
- Micrurus tener toxin partial de novo sequencing: Manual and automated de novo sequencing with CID and ETD data recovered the N-terminal half of a disulfide-bridged Texas coral snake toxin.
Methods it uses
- Manual MS/MS de novo interpretation: Protocol for manual de novo peptide sequencing from MS/MS spectra.
- PEAKS: Commercial DP-based de novo