Peptide de Novo Sequencing Facilitated by a Dual-Labeling Strategy
peer-reviewed · Analytical Chemistry · 2005
peer-reviewed · Analytical Chemistry · 2005. Richard L. Beardsley et al. A novel peptide derivatization strategy based on guanidination and amidination is presented. Mass-coded…
| Date | 2005-10-01 |
| Type | peer-reviewed |
| Venue | Analytical Chemistry |
| Publisher | American Chemical Society (ACS) |
| Contribution | adjacent |
| DOI | 10.1021/ac050540k |
| Citations (OpenAlex) | 48 |
| Venue 2-year citedness | 7.10 |
Abstract
A novel peptide derivatization strategy based on guanidination and amidination is presented. Mass-coded labels help distinguish N- and C-terminal fragment ions produced by collision-induced dissociation and are of general utility since peptide N-termini are coded. The amidine labels also promote specific fragmentation pathways that elucidate N-terminal residues and provide valuable internal calibrants. This strategy is demonstrated with the tryptic peptides of several model proteins, including two that are phosphorylated. Additionally, interpreted peptide sequences are matched against a database of over 80,000 proteins to assess the selectivity of this sequencing approach.
Methods and tools
- Dual-labeling (guanidination/amidination) de novo sequencing: Derivatizes peptides by guanidination and amidination so mass-coded labels distinguish N- and C-terminal fragment ions and promote informative fragmentation for de novo sequencing.