Peptide de Novo Sequencing Facilitated by a Dual-Labeling Strategy
peer-reviewed · Analytical Chemistry · 2005
peer-reviewed · Analytical Chemistry · 2005. Richard L. Beardsley et al. A novel peptide derivatization strategy based on guanidination and amidination is presented. Mass-coded…
| Date | 2005-10-01 |
| Type | peer-reviewed |
| Venue | Analytical Chemistry |
| Publisher | American Chemical Society (ACS) |
| Contribution | adjacent |
| DOI | 10.1021/ac050540k |
| Citations (OpenAlex) | 48 |
| Venue 2-year citedness | 7.10 |
Abstract
A novel peptide derivatization strategy based on guanidination and amidination is presented. Mass-coded labels help distinguish N- and C-terminal fragment ions produced by collision-induced dissociation and are of general utility since peptide N-termini are coded. The amidine labels also promote specific fragmentation pathways that elucidate N-terminal residues and provide valuable internal calibrants. This strategy is demonstrated with the tryptic peptides of several model proteins, including two that are phosphorylated. Additionally, interpreted peptide sequences are matched against a database of over 80,000 proteins to assess the selectivity of this sequencing approach.
Methods and tools
- Dual-labeling (guanidination/amidination) de novo sequencing: Derivatizes peptides by guanidination and amidination so mass-coded labels distinguish N- and C-terminal fragment ions and promote informative fragmentation for de novo sequencing.
Cites (7)
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Cited by (12)
- Enhancing De Novo Protein Sequencing through the C-Terminal Labeling Strategy: Resolving Isobaric Ambiguities by Electron-Transfer/Higher Energy Collision Dissociation (EThcD) (2024) crossref
- Optimization of carbamylation conditions and study on the effects on the product ions of carbamylation and dual modification of the peptide by Q-TOF MS (2018) crossref
- Bromine isotopic signature facilitates de novo sequencing of peptides in free‐radical‐initiated peptide sequencing (FRIPS) mass spectrometry (2015) crossref
- De Novo Sequencing of Peptides Using Selective 351 nm Ultraviolet Photodissociation Mass Spectrometry (2013) crossref
- 193 nm Ultraviolet Photodissociation of Imidazolinylated Lys-N Peptides for De Novo Sequencing (2012) crossref
- De novo analysis of protein N-terminal sequence utilizing MALDI signal enhancing derivatization with Br signature (2012) crossref
- C-terminal de novo sequencing of peptides using oxazolone-based derivatization with bromine signature (2011) crossref
- An effective method for de novo peptide sequencing based on phosphorylation strategy and mass spectrometry (2011) crossref
- Characterization of a peptide family from the skin secretion of the Middle East Tree Frog Hyla savignyi by composition‐based de novo sequencing (2010) crossref
- Peptide de Novo Sequencing Using 157 nm Photodissociation in a Tandem Time-of-Flight Mass Spectrometer (2010) crossref
- Simplifying Fragmentation Patterns of Multiply Charged Peptides by N-Terminal Derivatization and Electron Transfer Collision Activated Dissociation (2009) crossref
- Rapid and Reliable Peptide de Novo Sequencing Facilitated by Microfluidic Chip-Based Edman Degradation (2008) crossref