Proteomic profiling of Camelus dromedarius blood leukocytes using protein sequence database searches and de novo peptide sequencing
peer-reviewed · Journal of King Saud University – Science · 2026
| Date | 2026-10-08 |
| Type | peer-reviewed |
| Venue | Journal of King Saud University – Science |
| Publisher | Scientific Scholar |
| Contribution | downstream-application |
| DOI | 10.25259/jksus_108_2026 |
| Citations (OpenAlex) | 0 |
Abstract
There is a lack of studies on the crude leukocyte proteome of camels. Therefore, we hypothesized that camel leukocytes could be a source of bioactive peptides that have not been studied before. Here, we present the first comprehensive proteomic analysis of dromedary camel crude leukocyte-derived polypeptides (CLDP) using both protein sequence database searches (PSD) and de novo peptide sequencing (DPS) to improve peptide identification and coverage. The crude protein was extracted and analyzed by LC-MS/MS, followed by identification of protein and peptide sequences through PSD and DPS. PSD identified 120 proteins based on the Camelus dromedarius and Sus scrofa databases, while DPS identified a total of 830 peptides with an average local confidence (ALC) of 95% or higher. Gene Ontology (GO) annotation and KEGG analysis highlighted terms associated with stress adaptation, extracellular signaling, and immune response. However, DPS exhibited a broader and more pronounced enrichment trend, particularly in relation to the immune system.
Methods and tools
- Camel leukocyte peptidome: First proteomic survey of dromedary camel crude leukocyte polypeptides: PEAKS Studio de novo sequencing (ALC 95% or higher) gave 830 peptides beside the 120 proteins from database search, and the de novo peptides were the more strongly enriched for immune-system terms, as a source of candidate bioactive peptides.
Methods it uses
- PEAKS: Commercial DP-based de novo
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