Purification and Partial Characterization of a New Antitumor Protein from Tegillarca granosa
peer-reviewed · Marine Drugs · 2015
peer-reviewed · Marine Drugs · 2015. Shuangshuang Lv et al. A new protein, coded as D2-3, was obtained from the marine organism Tegillarca granosa L. by anion exchange…
| Date | 2015-03-17 |
| Type | peer-reviewed |
| Venue | Marine Drugs |
| Publisher | MDPI AG |
| Contribution | downstream-application |
| DOI | 10.3390/md13031466 |
| Citations (OpenAlex) | 16 |
Abstract
A new protein, coded as D2-3, was obtained from the marine organism Tegillarca granosa L. by anion exchange and hydrophobic chromatography. The purity of D2-3 was over 99.0% as measured by RP-HPLC. Its molecular weight was shown to be 20.320 kDa by ESI-MS/MS, and the isoelectric point of D2-3 was 4.70. The antitumor activity of D2-3 against four human tumor cell lines was measured by MTT assay. The conformational structure of D2-3 was further characterized by UV-vis, FT-IR and CD spectroscopy. Partial amino acid sequences of D2-3 were determined to be LMMTDVEESR, SSHMLSECRRK, KNGRNVDISHKDKG, SSDPTLMDPDDTNKDR, SSDKNTCSKTEYYTR and SSETMPYDVLDTNEMR via MALDI-TOF-MS and de novo sequencing.
Methods and tools
- Tegillarca granosa antitumor protein D2-3: Purification of an antitumor protein from the blood clam Tegillarca granosa, with six partial amino acid sequences determined by MALDI-TOF/TOF de novo sequencing.