R-met C-terminal labeling de novo sequencing
adjacent · Chemical labeling assisted
R-met C-terminal labeling de novo sequencing: adjacent · Chemical labeling assisted. Labels the peptide C-terminus and Asp/Glu carboxyl groups with arginine methyl ester so non-tryptic peptides fragment well, and EThcD then resolves isobaric…
Labels the peptide C-terminus and Asp/Glu carboxyl groups with arginine methyl ester so non-tryptic peptides fragment well, and EThcD then resolves isobaric ambiguities for bottom-up de novo protein sequencing.
| Kind | adjacent |
| Deep learning | no |
| Acquisition | DDA |
| Family | Chemical labeling assisted |
Paper describing it
- Enhancing De Novo Protein Sequencing through the C-Terminal Labeling Strategy: Resolving Isobaric Ambiguities by Electron-Transfer/Higher Energy Collision Dissociation (EThcD) (2024, Analytical Chemistry, peer-reviewed)