Enzymatic ladder assisted
2 methods · 2009–2012
Enzymatic ladder assisted: A protease chosen so that every peptide carries a predictable terminal residue, such as Lys-N, which turns the fragment ladder into a simpler series that is easier to read de novo.
A protease chosen so that every peptide carries a predictable terminal residue, such as Lys-N, which turns the fragment ladder into a simpler series that is easier to read de novo.
The earliest of its 2 methods is Lys-N MALDI-MS/MS de novo sequencing (2009).
| Methods | 2 |
| Papers describing them | 3 |
| Authors | 12 |
| Active | 2009-04-01 to 2012-01-10 |
| Kinds | adjacent (2) |
| Acquisition | DDA (2) |
Methods (2)
Oldest first, by the paper that describes each one.
- Lys-N MALDI-MS/MS de novo sequencing (2009): De novo peptide sequencing strategy using Lys-N metalloendopeptidase digestion with MALDI-MS/MS.
- Lys-N ETD database-independent proteomics (2012): Combines Lys-N digestion, ETD and CID/ETD cross-validation to de novo sequence thousands of peptides from the unsequenced ostrich proteome.
Papers describing them (3)
- Straightforward and de Novo Peptide Sequencing by MALDI-MS/MS Using a Lys-N Metalloendopeptidase (2009, Molecular & Cellular Proteomics, peer-reviewed)
- Lys-N: A versatile enzyme for proteomics (2010, thesis)
- Database independent proteomics analysis of the ostrich and human proteome (2012, Proceedings of the National Academy of Sciences, peer-reviewed)