Functional Proteome Analysis of the Banana Plant (Musa spp.) Using de Novo Sequence Analysis of Derivatized Peptides
peer-reviewed · Journal of Proteome Research · 2007
peer-reviewed · Journal of Proteome Research · 2007. Bart Samyn et al. We report the use of chemical derivatization with MALDI-MS/MS analysis for de novo sequence analysis. Using…
| Date | 2007-01-01 |
| Type | peer-reviewed |
| Venue | Journal of Proteome Research |
| Publisher | American Chemical Society (ACS) |
| Contribution | downstream-application |
| DOI | 10.1021/pr0601943 |
| Citations (OpenAlex) | 49 |
| Venue 2-year citedness | 3.83 |
Abstract
We report the use of chemical derivatization with MALDI-MS/MS analysis for de novo sequence analysis. Using three frequently used homology-based search algorithms, we were able to identify more than 40 proteins from banana, a nonmodel plant with unsequenced genome. Furthermore, this approach allowed the identification of different isoforms. We also observed that the identification score obtained varied according to the position of the peptide sequences in the query using the MS-Blast algorithm.
Methods and tools
- Banana proteome by de novo of derivatized peptides: Chemical derivatization and MALDI-TOF/TOF de novo sequencing with homology search (MS-BLAST etc.) identified more than 40 proteins and isoforms in banana, a non-model plant.