Identification of Antimicrobial Peptides from HAN-J6 Hemp Seeds Using Mass Spectrometry and De Novo Peptide Sequencing

abstract · Journal of Student-Scientists’ Research · 2026

abstract · Journal of Student-Scientists’ Research · 2026. Lauren Zhong et al.
Date 2026-09-24
Type abstract
Venue Journal of Student-Scientists’ Research
Publisher George Mason University
Contribution downstream-application
DOI 10.13021/jssr2026.5545

Authors

  1. Lauren Zhong · George Mason University
  2. Edward Bentil · George Mason University

Methods and tools

  • Hemp seed antimicrobial peptides: Screens HAN-J6 hemp seed (Cannabis sativa L.) protein for antimicrobial peptides, a source not previously characterised this way. Defatting, alkaline extraction and acid precipitation, then trypsin/Lys-C digestion and LC-MS; PEAKS 12 de novo sequencing returned 3,985 peptides, and CAMP R3 and AMP Scanner_V2 screened the ALC>=80% list to 56 and 260 candidates respectively, 27 of which both tools agreed on.

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