Large Scale Discovery and De Novo-Assisted Sequencing of Cationic Antimicrobial Peptides (CAMPs) by Microparticle Capture and Electron-Transfer Dissociation (ETD) Mass Spectrometry
peer-reviewed · Journal of Proteome Research · 2015
| Date | 2015-10-02 |
| Type | peer-reviewed |
| Venue | Journal of Proteome Research |
| Publisher | American Chemical Society (ACS) |
| Contribution | downstream-application |
| DOI | 10.1021/acs.jproteome.5b00447 |
| Citations (OpenAlex) | 29 |
| Venue 2-year citedness | 3.83 |
Abstract
The identification and sequencing of novel cationic antimicrobial peptides (CAMPs) have proven challenging due to the limitations associated with traditional proteomics methods and difficulties sequencing peptides present in complex biomolecular mixtures. We present here a process for large-scale identification and de novo-assisted sequencing of newly discovered CAMPs using microparticle capture followed by tandem mass spectrometry equipped with electron-transfer dissociation (ETD). This process was initially evaluated and verified using known CAMPs with varying physicochemical properties. The effective parameters were then applied in the analysis of a complex mixture of peptides harvested from American alligator plasma using custom-made (Bioprospector) functionalized hydrogel particles. Here, we report the successful sequencing process for CAMPs that has led to the identification of 340 unique peptides and the discovery of five novel CAMPs from American alligator plasma.
Methods and tools
- American alligator plasma CAMPs: Captured cationic antimicrobial peptides from American alligator plasma with functionalized hydrogel microparticles and sequenced them by de novo-assisted ETD tandem MS, identifying 340 peptides and five novel antimicrobials.
Methods it uses
- PEAKS: Commercial DP-based de novo