Math, science, history, unraveling the mystery—That all started with de novo!
peer-reviewed · EuPA Open Proteomics · 2019
peer-reviewed · EuPA Open Proteomics · 2019. Ekaterina Ilgisonis et al. This work on solving the mystery of words encoded by amino acids in peptides was derived by the YPIC-EuPA…
| Date | 2019-03-01 |
| Type | peer-reviewed |
| Venue | EuPA Open Proteomics |
| Publisher | Elsevier BV |
| Contribution | downstream-application |
| DOI | 10.1016/j.euprot.2019.07.011 |
| Citations (OpenAlex) | 0 |
Abstract
This work on solving the mystery of words encoded by amino acids in peptides was derived by the YPIC-EuPA Challenge. We received a dry synthetic peptide sample and performed a mass spectrometric analysis followed by de novo peptide sequencing. As a result, a part of “Rays of positive electricity and their application to chemical analyses” by J.J.Tomson was found to be encoded in the peptides of the sample. The words were first revealed from the peptides, that matched by Google search to find the answer. After that, the answer was validated using a standard proteomic search against a database constructed from the quotation found.
Methods and tools
- YPIC encoded-text de novo decoding (Thomson quote): De novo sequencing of synthetic peptides from the YPIC-EuPA challenge revealed a quotation from J.J. Thomson, later confirmed by database search.
Methods it uses
- PEAKS: Commercial DP-based de novo