Primary sequence determination of a monoclonal antibody against α-synuclein using a novel mass spectrometry-based approach
peer-reviewed · International Journal of Mass Spectrometry · 2012
peer-reviewed · International Journal of Mass Spectrometry · 2012. Eric Sousa et al.
| Date | 2012-02-01 |
| Type | peer-reviewed |
| Venue | International Journal of Mass Spectrometry |
| Publisher | Elsevier BV |
| Contribution | downstream-application |
| DOI | 10.1016/j.ijms.2011.05.005 |
| Citations (OpenAlex) | 11 |
Methods and tools
- Anti alpha-synuclein mAb sequencing: The primary sequence of a monoclonal antibody against alpha-synuclein was determined de novo by mass spectrometry combining ion-trap CID and HCD fragmentation.
Cites (6)
- pNovo: De novo Peptide Sequencing and Identification Using HCD Spectra (2010) crossref
- De novo sequencing of peptides by MS/MS (2010) crossref
- De novo peptide sequencing by tandem MS using complementary CID and electron transfer dissociation (2009) crossref
- Automated de novo protein sequencing of monoclonal antibodies (2008) crossref
- MSNovo: A Dynamic Programming Algorithm for de Novo Peptide Sequencing via Tandem Mass Spectrometry (2007) crossref
- De novo proteomic sequencing of a monoclonal antibody raised against OX40 ligand (2006) crossref
Cited by (7)
- Simultaneous polyclonal antibody sequencing and epitope mapping by cryo electron microscopy and mass spectrometry (2025) crossref
- A Handle on Mass Coincidence Errors in De Novo Sequencing of Antibodies by Bottom-up Proteomics (2024) both
- Template-Based Assembly of Proteomic Short Reads For De Novo Antibody Sequencing and Repertoire Profiling (2022) both
- Template-based assembly of proteomic short reads for de novo antibody sequencing and repertoire profiling (2022) crossref
- Mass Spectrometry-Based De Novo Sequencing of Monoclonal Antibodies Using Multiple Proteases and a Dual Fragmentation Scheme (2021) both
- Mass spectrometry-based de novo sequencing of the anti-FLAG-M2 antibody using multiple proteases and a dual fragmentation scheme (2021) both
- Combining phage display with de novo protein sequencing for reverse engineering of monoclonal antibodies (2016) both