De novo proteomic sequencing of a monoclonal antibody raised against OX40 ligand
peer-reviewed · Analytical Biochemistry · 2006
peer-reviewed · Analytical Biochemistry · 2006. Victoria Pham et al. De novo sequencing of a full-length monoclonal antibody raised against OX40 ligand is described. Using a…
| Date | 2006-05-01 |
| Type | peer-reviewed |
| Venue | Analytical Biochemistry |
| Publisher | Elsevier BV |
| Contribution | downstream-application |
| DOI | 10.1016/j.ab.2006.02.001 |
| Citations (OpenAlex) | 30 |
Abstract
De novo sequencing of a full-length monoclonal antibody raised against OX40 ligand is described. Using a combination of overlapping complementary proteolytic and chemical digestions, with analysis by mass spectrometry and Edman degradation, both the heavy and light chains were fully sequenced. Particular attention was paid to those modifications that could be susceptible to degradation in the complementarity determining region and Fc region. An overview of the protocol is described, and suggestions for improvements to aid in such sequencing projects in the future are discussed.
Methods and tools
- Anti-OX40L mAb de novo sequencing: Full-length de novo sequencing of a monoclonal antibody raised against OX40 ligand, from overlapping proteolytic and chemical digests read by mass spectrometry and cross-checked by Edman degradation.
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