Rapid and Reliable Peptide de Novo Sequencing Facilitated by Microfluidic Chip-Based Edman Degradation
peer-reviewed · Journal of Proteome Research · 2008
| Date | 2008-02-01 |
| Type | peer-reviewed |
| Venue | Journal of Proteome Research |
| Publisher | American Chemical Society (ACS) |
| Contribution | adjacent |
| DOI | 10.1021/pr070465p |
| Citations (OpenAlex) | 1 |
| Venue 2-year citedness | 3.83 |
Abstract
This paper expands the application of the newly developed highly sensitive microfluidic chip-based Edman degradation system. Comparison between the MS/MS spectra of a native peptide and its N-terminus truncated counterpart after carrying out one cycle of Edman degradation in a microfluidic chip can not only provide N-terminal residue information, but also facilitate the identification of different series of fragment ions. Manual peptide sequencing is more feasible and rapid using this method as demonstrated with three peptide examples including one neuropeptide. Furthermore, two cycles of Edman degradation allow the determination of the exact value of b 2 ion of the intact peptide, which can serve as an internal calibrant to increase the mass accuracy of the MS/MS spectrum.
Methods and tools
- Microfluidic Edman-assisted MS/MS de novo sequencing: Removes one or two N-terminal residues by on-chip Edman degradation and compares MS/MS spectra before and after, which identifies ion series and calibrates b2 for faster manual de novo sequencing.
Methods it uses
- Manual MS/MS de novo interpretation: Protocol for manual de novo peptide sequencing from MS/MS spectra.
Cites (8)
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