SearchGUI: A Highly Adaptable Common Interface for Proteomics Search and de Novo Engines
peer-reviewed · Journal of Proteome Research · 2018
peer-reviewed · Journal of Proteome Research · 2018. Harald Barsnes et al. Mass-spectrometry-based proteomics has become the standard approach for identifying and quantifying proteins…
| Date | 2018-05-18 |
| Type | peer-reviewed |
| Venue | Journal of Proteome Research |
| Publisher | American Chemical Society |
| Contribution | adjacent |
| DOI | 10.1021/acs.jproteome.8b00175 |
| Citations (OpenAlex) | 229 |
| Venue 2-year citedness | 3.48 |
Abstract
Mass-spectrometry-based proteomics has become the standard approach for identifying and quantifying proteins. A vital step consists of analyzing experimentally generated mass spectra to identify the underlying peptide sequences for later mapping to the originating proteins. We here present the latest developments in SearchGUI, a common open-source interface for the most frequently used freely available proteomics search and de novo engines that has evolved into a central component in numerous bioinformatics workflows.
Methods and tools
- SearchGUI: Open-source common interface wrapping a wide set of proteomics database search engines (X!Tandem, MS-GF+, Comet, OMSSA, MS Amanda, MyriMatch, Tide, Andromeda, MetaMorpheus, Sage) plus classical de novo engines (PepNovo+, DirecTag, Novor, pNovo). Sister project to DeNovoGUI from the same CompOmics-aligned Bergen / Ghent group.
Cites (4)
- Novor: Real-Time Peptide de Novo Sequencing Software (2015) crossref
- PeptideShaker enables reanalysis of MS-derived proteomics data sets (2015) crossref
- Lessons in de novo peptide sequencing by tandem mass spectrometry (2015) crossref
- DirecTag: Accurate Sequence Tags from Peptide MS/MS through Statistical Scoring (2008) crossref
Cited by (3)
- CasanovoGUI: a cross-platform desktop application for deep learning-based de novo peptide sequencing with Casanovo (2026) both
- Comprehensive evaluation of peptide de novo sequencing tools for monoclonal antibody assembly (2023) both
- Extended Snake Venomics by Top-Down In-Source Decay: Investigating the Newly Discovered Anatolian Meadow Viper Subspecies, Vipera anatolica senliki (2020) both