Shotgun Protein Sequencing
algorithm · Spectral assembly
Shotgun Protein Sequencing: algorithm · Spectral assembly. Assembles overlapping MS/MS spectra of related peptides into contigs before any sequence is called, so a protein is sequenced from the assembly rather than from…
Assembles overlapping MS/MS spectra of related peptides into contigs before any sequence is called, so a protein is sequenced from the assembly rather than from individual spectra. Tolerates mixtures and unanticipated modifications.
| Kind | algorithm |
| Deep learning | no |
| Acquisition | DDA |
| Also known as | SPS |
| Family | Spectral assembly |
Papers describing it (4)
- Shotgun Protein Sequencing by Tandem Mass Spectra Assembly (2004, Analytical Chemistry, peer-reviewed)
- Protein identification by spectral networks analysis (2007, Proceedings of the National Academy of Sciences, peer-reviewed)
- Spectral Networks: A New Approach to De Novo Discovery of Protein Sequences and Posttranslational Modifications (2007, BioTechniques, peer-reviewed)
- Shotgun Protein Sequencing: Assembly of Peptide Tandem Mass Spectra from Mixtures of Modified Proteins (2007, Molecular & Cellular Proteomics, peer-reviewed)
Paper using it
An application or evaluation that ran this method. It is not counted among its authors below.
- Automated de novo protein sequencing of monoclonal antibodies (2008, Nature Biotechnology, peer-reviewed)