“De Novo” Sequencing of Peptides Recovered from In-Gel Digested Proteins by Nanoelectrospray Tandem Mass Spectrometry
peer-reviewed · Molecular Biotechnology · 2003
peer-reviewed · Molecular Biotechnology · 2003. Andrej Shevchenko et al. Proteins separated by one-dimensional or two-dimensional gel electrophoresis can be digested in-gel with…
| Date | 2003-11-17 |
| Type | peer-reviewed |
| Venue | Molecular Biotechnology |
| Publisher | Springer Science and Business Media LLC |
| Contribution | algorithm |
| DOI | 10.1385/mb:20:1:107 |
| Citations (OpenAlex) | 91 |
| Venue 2-year citedness | 3.96 |
Abstract
Proteins separated by one-dimensional or two-dimensional gel electrophoresis can be digested in-gel with trypsin and the recovered peptides can be sequenced de novo using triple quadrupole or hybrid quadrupole time-of-flight instruments equipped with a nanoelectrospray ion source. The peptide sequences determined provide useful information for identification of proteins by homology searching for cloning of the cognate genes by PCR based approaches.
Methods and tools
- Rapid QTOF de novo sequencing: Nanoelectrospray, isotopic-labeling, and quadrupole/time-of-flight workflow for rapid de novo peptide sequencing.