De Novo Sequencing of Peptides Using Mass Spectrometry
peer-reviewed · Wiley Encyclopedia of Chemical Biology · 2009
| Date | 2009-03-13 |
| Type | peer-reviewed |
| Venue | Wiley Encyclopedia of Chemical Biology |
| Publisher | Wiley |
| Contribution | review |
| DOI | 10.1002/9780470048672.wecb720 |
| Citations (OpenAlex) | 0 |
Abstract
Peptides are important biomolecules that play a variety of functional roles. Knowledge of the specific sequence of amino acids in peptides is essential to understand their biological functions, to identify them in target tissues, to map changes in their metabolic profile, to assess the purity of newly synthesized peptides and to characterize their precursor proteins. Currently, mass spectrometry has assumed a pivotal role in determining the sequence of peptides. The procedure involves conversion of peptide molecules into gas‐phase ions, fragmentation of these species into the sequence‐specific ions, and deciphering the mass spectral data into the sequence of the target peptide. This procedure, known as “ de novo sequencing”, requires understanding of the peptide fragmentation rules and guidelines for interpretation of a mass spectrum. These aspects are discussed in detail in this chapter.
Methods and tools
- De novo sequencing of peptides (Wiley Encyclopedia of Chemical Biology): Reference review of de novo peptide sequencing by mass spectrometry: ionization, fragmentation rules and how to interpret a spectrum into a sequence.