Mass Spectrometric De Novo Sequencing of Natural Peptides
peer-reviewed · Methods in Molecular Biology · 2024
peer-reviewed · Methods in Molecular Biology · 2024. Irina D. Vasileva et al. Natural peptides secreted under stress conditions by many organisms are bioactive molecules with a broad…
| Date | 2024-03-29 |
| Type | peer-reviewed |
| Venue | Methods in Molecular Biology |
| Publisher | Springer US |
| Contribution | downstream-application |
| DOI | 10.1007/978-1-0716-3646-6_3 |
| Citations (OpenAlex) | 1 |
| Venue 2-year citedness | 1.14 |
Abstract
Natural peptides secreted under stress conditions by many organisms are bioactive molecules with a broad spectrum of activities. These molecules could become potential models for novel pharmaceuticals, to which bacteria, according to modern scientific concepts, do not have and cannot develop resistance. Taking this into consideration, it is necessary to clarify the amino acid sequences of such peptides. Here we describe our approach to de novo sequencing of amphibians’ skin secretion peptides.
Methods and tools
- Amphibian skin peptide de novo protocol: Methods in Molecular Biology protocol for the mass spectrometric de novo sequencing of the skin secretion peptides of amphibians, from the Lebedev group.