Mass Spectrometric De Novo Sequencing of Natural Peptides

peer-reviewed · Methods in Molecular Biology · 2024

peer-reviewed · Methods in Molecular Biology · 2024. Irina D. Vasileva et al. Natural peptides secreted under stress conditions by many organisms are bioactive molecules with a broad…
Date 2024-03-29
Type peer-reviewed
Venue Methods in Molecular Biology
Publisher Springer US
Contribution downstream-application
DOI 10.1007/978-1-0716-3646-6_3
Citations (OpenAlex) 2
Venue 2-year citedness 1.14

Abstract

Natural peptides secreted under stress conditions by many organisms are bioactive molecules with a broad spectrum of activities. These molecules could become potential models for novel pharmaceuticals, to which bacteria, according to modern scientific concepts, do not have and cannot develop resistance. Taking this into consideration, it is necessary to clarify the amino acid sequences of such peptides. Here we describe our approach to de novo sequencing of amphibians’ skin secretion peptides.

Authors

  1. Irina D. Vasileva · Lomonosov Moscow State University, MSU-BIT University
  2. Tatiana Yu. Samgina · Lomonosov Moscow State University, MSU-BIT University
  3. Albert T. Lebedev · Lomonosov Moscow State University, MSU-BIT University

Methods and tools

  • Amphibian skin peptide de novo protocol: Methods in Molecular Biology protocol for the mass spectrometric de novo sequencing of the skin secretion peptides of amphibians, from the Lebedev group.

Cites (7)

Cited by (1)

Seen in the charts

Back to the full map

Back to top