Peptidomics of Acanthoscurria gomesiana spider venom reveals new toxins with potential antimicrobial activity
peer-reviewed · Journal of Proteomics · 2017
| Date | 2017-01-01 |
| Type | peer-reviewed |
| Venue | Journal of Proteomics |
| Publisher | Elsevier BV |
| Contribution | downstream-application |
| DOI | 10.1016/j.jprot.2016.07.012 |
| Citations (OpenAlex) | 51 |
| Venue 2-year citedness | 3.02 |
Abstract
Acanthoscurria gomesiana is a Brazilian spider from the Theraphosidae family inhabiting regions of Southeastern Brazil. Potent antimicrobial peptides as gomesin and acanthoscurrin have been discovered from the spider hemolymph in previous works. Spider venoms are also recognized as sources of biologically active peptides, however the venom peptidome of A. gomesiana remained unexplored to date. In this work, a MS-based workflow was applied to the investigation of the spider venom peptidome. Data-independent and data-dependent LC-MS/MS acquisitions of intact peptides and of peptides submitted to multiple enzyme digestions, followed by automated chromatographic alignment, de novo analysis, database and homology searches with manual validations showed that the venom is composed by Biological significance Biological fluids of the Acanthoscurria gomesiana spider are sources of active molecules, as is the case of antimicrobial peptides and acylpolyamines found in the hemolymphs. The venom is also a potential source of toxins with pharmacological and biotechnological applications. However, to our knowledge no A. gomesiana venom toxin structure has been determined to date. Using a combination of high resolution mass spectrometry, transcriptomics and bioinformatics, we employed a workflow to fully sequence, determine the number of disulfide bonds of mature peptides and we found new potential antimicrobial peptides. This workflow is suitable for complete peptide toxin sequencing when handling limited amount of venom samples and can accelerate the discovery of peptides with potential biotechnological applications.
Methods and tools
- Acanthoscurria gomesiana venom peptidomics: MS-based peptidomics of the Brazilian tarantula Acanthoscurria gomesiana venom, using de novo analysis plus transcriptome and homology searches to fully sequence mature cysteine-rich toxins and find new antimicrobial peptides.
Methods it uses
Data deposited
- Peptidomics of Acanthoscurria gomesiana spider venom reveals new toxins with potential antimicrobial activity — as deposited · PXD003884
Cites (6)
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