Proteomics of Multigenic Families from Species Underrepresented in Databases: The Case of Loquat (Eriobotrya japonica Lindl.) Polyphenol Oxidases
peer-reviewed · Journal of Proteome Research · 2008
peer-reviewed · Journal of Proteome Research · 2008. Susana Sellés-Marchart et al. Here, we approach the problem of obtaining accurate and reliable information about the gene origin of a…
| Date | 2008-09-05 |
| Type | peer-reviewed |
| Venue | Journal of Proteome Research |
| Publisher | American Chemical Society (ACS) |
| Contribution | downstream-application |
| DOI | 10.1021/pr700687c |
| Citations (OpenAlex) | 16 |
| Venue 2-year citedness | 3.83 |
Abstract
Here, we approach the problem of obtaining accurate and reliable information about the gene origin of a protein belonging to a multigenic family, polyphenol oxidase, from an underrepresented species, Eriobotrya japonica. De novo sequencing was a key approach to obtain broad sequence coverage. Alignment of peptides on their most similar homologous protein revealed divergent amino acid positions that lead to hypothesize the minimal number of genes encoding for the proteins analyzed.
Methods and tools
- Loquat polyphenol oxidase isoforms: De novo sequencing of polyphenol oxidases from loquat, a database-underrepresented species, aligned to homologues to infer the minimal number of encoding genes.