Q-Tof ESI-MS/MS and automated software interpretation for de novo sequencing of tryptic peptides from gel separated proteins

abstract · Biochemical Society Transactions · 2000

abstract · Biochemical Society Transactions · 2000. D. Gostick et al. Conference Abstract| October 01 2000 Q-Tof ESI-MS/MS and automated software interpretation for de novo…
Date 2000-10-01
Type abstract
Venue Biochemical Society Transactions
Publisher Portland Press Ltd.
Contribution algorithm
DOI 10.1042/bst028a260a
Citations (OpenAlex) 0

Abstract

Conference Abstract| October 01 2000 Q-Tof ESI-MS/MS and automated software interpretation for de novo sequencing of tryptic peptides from gel separated proteins D. Gostick; D. Gostick 1Micromass UK Ltd, Wythemhawe, Manchester, M23 9LZ, UK Search for other works by this author on: This Site PubMed Google Scholar J. Langridge; J. Langridge 1Micromass UK Ltd, Wythemhawe, Manchester, M23 9LZ, UK Search for other works by this author on: This Site PubMed Google Scholar R. Rachubinski; R. Rachubinski 2University of Alberta, Dept of Cell Biology, Edmonton, Canada, T6G 2HZ. 3MaxEnt Solutions, Cambridge CB3 7HJ, UK Search for other works by this author on: This Site PubMed Google Scholar R. Wozniak; R. Wozniak 2University of Alberta, Dept of Cell Biology, Edmonton, Canada, T6G 2HZ. 3MaxEnt Solutions, Cambridge CB3 7HJ, UK Search for other works by this author on: This Site PubMed Google Scholar J. Smith; J. Smith 2University of Alberta, Dept of Cell Biology, Edmonton, Canada, T6G 2HZ. 3MaxEnt Solutions, Cambridge CB3 7HJ, UK Search for other works by this author on: This Site PubMed Google Scholar J. Cottrell; J. Cottrell 1Micromass UK Ltd, Wythemhawe, Manchester, M23 9LZ, UK Search for other works by this author on: This Site PubMed Google Scholar J. Hoyes; J. Hoyes 1Micromass UK Ltd, Wythemhawe, Manchester, M23 9LZ, UK Search for other works by this author on: This Site PubMed Google Scholar E. Kapp; E. Kapp 1Micromass UK Ltd, Wythemhawe, Manchester, M23 9LZ, UK Search for other works by this author on: This Site PubMed Google Scholar J. Skilling; J. Skilling 2University of Alberta, Dept of Cell Biology, Edmonton, Canada, T6G 2HZ. 3MaxEnt Solutions, Cambridge CB3 7HJ, UK Search for other works by this author on: This Site PubMed Google Scholar R. Bordoli R. Bordoli 1Micromass UK Ltd, Wythemhawe, Manchester, M23 9LZ, UK Search for other works by this author on: This Site PubMed Google Scholar Biochem Soc Trans (2000) 28 (5): A260. https://doi.org/10.1042/bst028a260a Views Icon Views Article contents Figures & tables Video Audio Supplementary Data Peer Review Share Icon Share MailTo Twitter LinkedIn Cite Icon Cite Get Permissions Citation D. Gostick, J. Langridge, R. Rachubinski, R. Wozniak, J. Smith, J. Cottrell, J. Hoyes, E. Kapp, J. Skilling, R. Bordoli; Q-Tof ESI-MS/MS and automated software interpretation for de novo sequencing of tryptic peptides from gel separated proteins. Biochem Soc Trans 1 October 2000; 28 (5): A260. doi: https://doi.org/10.1042/bst028a260a Download citation file: Ris (Zotero) Reference Manager EasyBib Bookends Mendeley Papers EndNote RefWorks BibTex toolbar search Search Dropdown Menu toolbar search search input Search input auto suggest filter your search All ContentAll JournalsBiochemical Society Transactions Search Advanced Search This content is only available as a PDF. © 2000 Biochemical Society2000 Article PDF first page preview Close Modal You do not currently have access to this content.

Authors

  1. D. Gostick
  2. J. Langridge
  3. R. Rachubinski · University of Alberta
  4. R. Wozniak · University of Alberta
  5. J. Smith · University of Alberta
  6. J. Cottrell
  7. J. Hoyes
  8. E. Kapp
  9. J. Skilling · University of Alberta
  10. R. Bordoli

Methods and tools

  • PepSeq: Interactive de novo peptide sequencing application in BioLynx, the biopolymer analysis component of the MassLynx suite. The manual is explicit that it is user-driven rather than automatic: PepSeq “has been designed as an interactive program that allows the user to make decisions at each step in deducing the sequence of a peptide”. It scores and annotates spectra on the a, b, y and z ions, and takes input either from a MassLynx data file or as a plain mass and intensity list. Three modes: typing a candidate sequence to see its theoretical fragments annotated against the spectrum; FindTag, which builds a set of sub-sequences whose series ions best match the spectrum and extends them step or leap-wise until the molecular weight matches the user-supplied precursor; and MassSeq, a separately purchased option that performs the sequencing automatically from the precursor mass, modifications and a mass-accuracy estimate. There is no methods paper: the method is documented only in the vendor manual, which is catalogued here as the accompanying resource. Provenance runs Micromass UK Ltd to Waters Corporation, and the Version 4.0 guide catches the handover in progress, carrying a Waters part number and a Micromass part number side by side over a 1993-2002 Micromass UK Ltd copyright.

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