Secondary Losses via γ-Lactam Formation in Hot Electron Capture Dissociation: A Missing Link to Complete de Novo Sequencing of Proteins?

peer-reviewed · Journal of the American Chemical Society · 2003

peer-reviewed · Journal of the American Chemical Society · 2003. Frank Kjeldsen et al. Differentiating between the isomeric isoleucine and leucine amino acid residues can be accomplished by a…
Date 2003-06-01
Type peer-reviewed
Venue Journal of the American Chemical Society
Publisher American Chemical Society (ACS)
Contribution adjacent
DOI 10.1021/ja035042c
Citations (OpenAlex) 40

Abstract

Differentiating between the isomeric isoleucine and leucine amino acid residues can be accomplished by a gas-phase fragmentation reaction in which an N-terminal radical site of a C-terminal fragment initiates formation of a five-membered ring with the beta-carbon in the adjacent residue.

Authors

  1. Frank Kjeldsen · University of Southern Denmark, Uppsala University
  2. Roman A. Zubarev · Cornell University, Karolinska Institutet, University of Southern Denmark, Uppsala University

Methods and tools

  • Hot ECD w-ion Leu/Ile discrimination: Uses secondary side-chain losses in hot electron capture dissociation, formed through a gamma-lactam ring, to tell isoleucine from leucine, which ordinary de novo sequencing cannot do.

Cites (2)

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