A learned score function improves the power of mass spectrometry database search
preprint · bioRxiv · 2024
| Date | 2024-01-26 |
| Type | preprint |
| Venue | bioRxiv |
| Publisher | Cold Spring Harbor Laboratory |
| Contribution | adjacent |
| DOI | 10.1101/2024.01.26.577425 |
| Citations (OpenAlex) | 3 |
Abstract
One of the core problems in the analysis of protein tandem mass spectrometry data is the peptide assignment problem: determining, for each observed spectrum, the peptide sequence that was responsible for generating the spectrum. Two primary classes of methods are used to solve this problem: database search and de novo peptide sequencing. State-of-the-art methods for de novo sequencing employ machine learning methods, whereas most database search engines use hand-designed score functions to evaluate the quality of a match between an observed spectrum and a candidate peptide from the database. We hypothesize that machine learning models for de novo sequencing implicitly learn a score function that captures the relationship between peptides and spectra, and thus may be re-purposed as a score function for database search. Because this score function is trained from massive amounts of mass spectrometry data, it could potentially outperform existing, hand-designed database search tools. To test this hypothesis, we re-engineered Casanovo, which has been shown to provide state-of-the-art de novo sequencing capabilities, to assign scores to given peptide-spectrum pairs. We then evaluated the statistical power of this Casanovo score function, Casanovo-DB, to detect peptides on a benchmark of three mass spectrometry runs from three different species. Our results show that, at a 1% peptide-level false discovery rate threshold, Casanovo-DB outperforms existing hand-designed score functions by 35% to 88%. In addition, we show that re-scoring with the Percolator post-processor benefits Casanovo-DB more than other score functions, further increasing the number of detected peptides.
Methods and tools
- Casanovo-DB: Database search scoring
Cites (4)
- PGPointNovo: an efficient neural network-based tool for parallel de novo peptide sequencing (2023) crossref
- De novo mass spectrometry peptide sequencing with a transformer model (2022) crossref
- pNovo 3: precise de novo peptide sequencing using a learning-to-rank framework (2019) crossref
- De novo peptide sequencing by deep learning (2017) crossref