A multi-protease, multi-dissociation, bottom-up-to-top-down proteomic view of the Loxosceles intermedia venom

peer-reviewed · Scientific Data · 2017

peer-reviewed · Scientific Data · 2017. Dilza Trevisan-Silva et al. Venoms are a rich source for the discovery of molecules with biotechnological applications, but their…
Date 2017-07-11
Type peer-reviewed
Venue Scientific Data
Publisher Springer Science and Business Media LLC
Contribution downstream-application
DOI 10.1038/sdata.2017.90
Citations (OpenAlex) 27
Venue 2-year citedness 5.72

Abstract

Venoms are a rich source for the discovery of molecules with biotechnological applications, but their analysis is challenging even for state-of-the-art proteomics. Here we report on a large-scale proteomic assessment of the venom of Loxosceles intermedia, the so-called brown spider. Venom was extracted from 200 spiders and fractioned into two aliquots relative to a 10 kDa cutoff mass. Each of these was further fractioned and digested with trypsin (4 h), trypsin (18 h), pepsin (18 h), and chymotrypsin (18 h), then analyzed by MudPIT on an LTQ-Orbitrap XL ETD mass spectrometer fragmenting precursors by CID, HCD, and ETD. Aliquots of undigested samples were also analyzed. Our experimental design allowed us to apply spectral networks, thus enabling us to obtain meta-contig assemblies, and consequently de novo sequencing of practically complete proteins, culminating in a deep proteome assessment of the venom. Data are available via ProteomeXchange, with identifier PXD005523.

Authors

  1. Dilza Trevisan-Silva · Universidade Federal do Paraná
  2. Aline V. Bednaski · Universidade Federal do Paraná
  3. Juliana S.G. Fischer · Fundação Oswaldo Cruz
  4. Silvio S. Veiga · Universidade Federal do Paraná
  5. Nuno Bandeira · University of California San Diego, University of California System
  6. Adrian Guthals · Abterra Biosciences (United States), Mapp Biopharmaceutical (United States), Mapp Biopharmaceutical, Inc., University of California San Diego, University of California System
  7. Fabricio K. Marchini · Fundação Oswaldo Cruz
  8. Felipe V. Leprevost · Fundação Oswaldo Cruz, University of Michigan
  9. Valmir C. Barbosa · Universidade Federal do Rio de Janeiro
  10. Andrea Senff-Ribeiro · Universidade Federal do Paraná
  11. Paulo C. Carvalho · Carlos Chagas Institute, Fiocruz Paraná, Fundação Oswaldo Cruz, University of California San Diego

Methods and tools

  • Loxosceles intermedia venom proteome: Multi-protease, multi-dissociation proteomics of brown spider venom, with spectral networks and meta-contig assembly yielding de novo sequences of nearly complete proteins.

Data deposited

  • A multi-protease, multi-dissociation, bottom-up-to-top-down proteomic view of the Loxosceles intermedia venom (as deposited) · PXD005523

Cites (6)

Cited by (4)

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