De Novo Interpretation of Tandem Mass Spectra
peer-reviewed · Current Protocols in Bioinformatics · 2009
peer-reviewed · Current Protocols in Bioinformatics · 2009. Bin Ma et al. De novo sequencing is an effective method for identifying unknown peptide sequences from their tandem mass…
| Date | 2009-03-01 |
| Type | peer-reviewed |
| Venue | Current Protocols in Bioinformatics |
| Publisher | Wiley |
| Contribution | review |
| DOI | 10.1002/0471250953.bi1310s25 |
| Citations (OpenAlex) | 11 |
Abstract
De novo sequencing is an effective method for identifying unknown peptide sequences from their tandem mass spectra. This unit briefly introduces how this can be done manually. A protocol for using the PEAKS online software for automated de novo sequencing is described. Finally, we show how to use the PEAKS scores to validate the de novo sequencing results.
Methods and tools
- De novo interpretation protocol (Current Protocols): A protocol unit explaining manual de novo interpretation of MS/MS spectra and how to run and validate automated de novo sequencing with PEAKS.
Methods it uses
- PEAKS: Commercial DP-based de novo