De Novo Sequencing Analysis of a Linear Peptide in the Venom of the Scorpion Buthacus leptochelys
peer-reviewed · Journal of the Mass Spectrometry Society of Japan · 2021
| Date | 2021-06-01 |
| Type | peer-reviewed |
| Venue | Journal of the Mass Spectrometry Society of Japan |
| Publisher | The Mass Spectrometry Society of Japan |
| Contribution | downstream-application |
| DOI | 10.5702/massspec.s21-04 |
| Citations (OpenAlex) | 0 |
Abstract
Scorpions utilize venom to capture prey and protect themselves from predators by showing neurotoxicity against insects and mammals. The scorpion venom also contains various components such as inorganic salts, nucleic acids, lipids, peptides, and proteins. Among them, peptides are main components responsible for the biological activities of the venom. Most of these peptides contain multiple disulfide bonds, but linear peptides without disulfide bonds are also present in the venom, which often exhibit antimicrobial activity. Mass spectrometry plays an important role in the structure determination of these peptides. In this paper, we describe the structural determination of a linear peptide from the venom of the North African scorpion Buthacus leptochelys using de novo sequencing analysis.
Methods and tools
- Buthacus leptochelys venom linear peptide: De novo structure determination of a linear, disulfide-free peptide from the venom of the scorpion Buthacus leptochelys.