Evolutionary instability of CUG-Leu in the genetic code of budding yeasts
peer-reviewed · Nature Communications · 2018
| Date | 2018-05-14 |
| Type | peer-reviewed |
| Venue | Nature Communications |
| Publisher | Springer Science and Business Media LLC |
| Contribution | downstream-application |
| DOI | 10.1038/s41467-018-04374-7 |
| Citations (OpenAlex) | 118 |
| Venue 2-year citedness | 17.60 |
Abstract
The genetic code used in nuclear genes is almost universal, but here we report that it changed three times in parallel during the evolution of budding yeasts. All three changes were reassignments of the codon CUG, which is translated as serine (in 2 yeast clades), alanine (1 clade), or the ‘universal’ leucine (2 clades). The newly discovered Ser2 clade is in the final stages of a genetic code transition. Most species in this clade have genes for both a novel tRNA Ser (CAG) and an ancestral tRNA Leu (CAG) to read CUG, but only tRNA Ser (CAG) is used in standard growth conditions. The coexistence of these alloacceptor tRNA genes indicates that the genetic code transition occurred via an ambiguous translation phase. We propose that the three parallel reassignments of CUG were not driven by natural selection in favor of their effects on the proteome, but by selection to eliminate the ancestral tRNA Leu (CAG).
Methods and tools
- Budding yeast CUG codon reassignment: Determined the translation of the CUG codon in budding yeast species from de novo peptide sequences and proteome database matching, revealing three parallel genetic code changes.
Methods it uses
- PEAKS: Commercial DP-based de novo
Data deposited
- Evolutionary Instability of CUG-Leu in the Genetic Code of Budding Yeasts — as deposited · PXD008827