Spectra pair
4 methods · 2009–2020
Spectra pair: Two spectra of the same peptide, from complementary fragmentation or from a paired digest, interpreted together so that one ion series fills the gaps in the other.
Two spectra of the same peptide, from complementary fragmentation or from a paired digest, interpreted together so that one ion series fills the gaps in the other.
The earliest of its 4 methods is CompNovo (2009); 3 more have followed.
| Methods | 4 |
| Papers describing them | 4 |
| Authors | 31 |
| Active | 2009-11-01 to 2020-02-15 |
| Kinds | algorithm (4) |
| Acquisition | DDA (4) |
Methods (4)
Oldest first, by the paper that describes each one.
- CompNovo (2009): De novo sequencing algorithm combining complementary CID and ETD tandem MS spectra.
- ADEPTS (2010): Advanced peptide de novo sequencing framework using a pair of tandem mass spectra.
- pNovoM (2019): De novo sequencing from mirror spectrum pairs: digesting the same sample with Ac-LysargiNase and with trypsin yields two spectra whose b and y ion series complement each other, which pins down the sequence far more confidently than either alone.
- Lys-Sequencer (2020): Database-independent de novo sequencing from paired Lys-C and Lys-N digests, whose single-residue-transposed peptides yield complementary high-resolution spectra, with residue-level confidence reported per call.
Papers describing them (4)
- De novo peptide sequencing by tandem MS using complementary CID and electron transfer dissociation (2009, Electrophoresis, peer-reviewed)
- ADEPTS: Advanced peptide de novo sequencing with a pair of tandem mass spectra (2010, Journal of Bioinformatics and Computational Biology, peer-reviewed)
- Precision De Novo Peptide Sequencing Using Mirror Proteases of Ac-LysargiNase and Trypsin for Large-scale Proteomics (2019, Molecular & Cellular Proteomics, peer-reviewed)
- Lys-Sequencer: An algorithm for de novo sequencing of peptides by paired single residue transposed Lys-C and Lys-N digestion coupled with high-resolution mass spectrometry (2020, Rapid Communications in Mass Spectrometry, peer-reviewed)