Cinerascetins, New Peptides fromHypsiboas cinerascens: MALDI LIFT-TOF-MS/MSde novoSequence and Imaging Analysis
peer-reviewed · Journal of the Brazilian Chemical Society · 2015
| Date | 2015-08-28 |
| Type | peer-reviewed |
| Venue | Journal of the Brazilian Chemical Society |
| Publisher | Sociedade Brasileira de Quimica (SBQ) |
| Contribution | downstream-application |
| DOI | 10.5935/0103-5053.20150219 |
| Citations (OpenAlex) | 6 |
Abstract
The continuous search for antimicrobial candidates pushes the pursuit of compounds in the most diverse organisms. Amphibians are known as a prolific source of antibacterial peptides. Based on the rich biodiversity of the Amazon region the unexplored green-tree frog (Hypsiboas cinerascens) was studied for its skin secretion peptide content. Chromatographic separations and established tandem mass spectrometry (MS/MS) methods were used for sequencing the primary structures of the purified compounds. De novo sequencing lead to the identification of five new peptides related to hylaseptin P1, displaying an aminated C-terminal. Sequencing of the complementary deoxyribonucleic acid (cDNA) analysis allowed the disambiguation of isobaric amino-acids for C-01. Matrix assisted laser desorption ionization (MALDI) was carried out, demonstrating the in situ co-occurrence of the identified peptides in the dorsal skin. The major peptide C-01 was synthesized and assayed against a selection of microorganisms displaying minimal inhibitory concentrations (MICs) ranging from 4 to 16 µM.
Methods and tools
- Hypsiboas cinerascens cinerascetins: De novo MALDI-TOF/TOF sequencing of skin secretion peptides of the green-tree frog Hypsiboas cinerascens revealed five new hylaseptin-related peptides, cinerascetins, also mapped by MALDI imaging.
Methods it uses
- Manual MS/MS de novo interpretation: Protocol for manual de novo peptide sequencing from MS/MS spectra.